Description
Human TJP1 Recombinant Protein (N-His)
Human TJP1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Val190, with an N-terminal His tag. Predicted molecular weight: 23.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Tight junction protein 1 (TJP1) is a ~195 kDa protein. TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. For research use only.
Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
| Product Name: | Human TJP1 Recombinant Protein (N-His) |
| SKU: | GXRP02084 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | TJP1, ZO1, Zonula occludens protein 1, Zona occludens protein 1, Tight junction protein ZO-1, Tight junction protein 1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Val190 |
| Calculated MW: | 23.03 kDa |
| UniProt: | Q07157 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
