The Human TLR2 (Toll-Like Receptor 2) ELISA Kit is specifically designed for the accurate detection of TLR2 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TLR2 is a key protein involved in the innate immune response, recognizing pathogens and activating the immune system. Dysregulation of TLR2 has been implicated in inflammatory diseases, autoimmune disorders, and infectious diseases, highlighting its importance as a biomarker in studying these conditions and developing targeted therapies. Overall, the Human TLR2 ELISA Kit from Assaygenie provides researchers with a powerful tool to investigate the role of TLR2 in health and disease, offering valuable insights into immune responses and potential therapeutic interventions.
Product Name:
Human TLR2 (Toll-Like Receptor 2) ELISA Kit
SKU:
HUES02074
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
