Description
Human TP53BP1 Recombinant Protein (N-His)
Human TP53BP1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala1614-His1972, with an N-terminal His tag. Predicted molecular weight: 41.86 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. TP53-binding protein 1 (TP53BP1) is a ~213 kDa protein. Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. For research use only.
Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs.
| Product Name: | Human TP53BP1 Recombinant Protein (N-His) |
| SKU: | GXRP02138 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | TP53-binding protein 1, TP53BP1, p53-binding protein 1, p53BP1, 53BP1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala1614-His1972 |
| Calculated MW: | 41.86 kDa |
| UniProt: | Q12888 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
