Human TrkB /NTRK2 (ZEB85) Antibody is a research-grade biosimilar monoclonal antibody. Format: scFv-Fc (human host). Reactivity: Human. Supplied as a liquid in 0.01M PBS pH 7.4. Applications: ELISA, Western blot, flow cytometry, functional assays and in vivo research. Endotoxin: < 10 EU/mg. Purity: >95% purity as determined by SDS-PAGE. For research use only.
BDNF/NT-3 growth factors receptor (TrkB/NTRK2) is a ~91 kDa protein. Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades.
Product Name:
Human TrkB /NTRK2 (ZEB85) Antibody
SKU:
GXBS01951
Size:
100 µg, 1 mg
Antibody Type:
Monoclonal Antibody
Clone:
ZEB85
Target:
Neurotrophic tyrosine kinase receptor type 2
Isotype:
scFv-Fc
Host Species:
Human
Reactivity:
Human
Applications:
ELISA, WB, FCM, Functional assay, Research in vivo
Expression Host:
Mammalian cells
Synonyms:
ZEB85
Recommended Dilutions:
ELISA: 1:2000-1:20000; WB: 1:500-1:2000
Biological Activity:
ZEB85 (10-100 nM; 4 days) increases neurite complexity in developing primary mouse hippocampal neurons, including neurite intersections, branch points and total length [1]. ZEB85 (100 nM; 21 days) fully rescues the reduced dendritic complexity of BDNF-deficient primary mouse hippocampal PV+ interneurons and increases the dendritic complexity of BDNF-sufficient PV+ interneurons [1]. ZEB85 (10 nM; 24 h) shifts the dendritic spine type distribution of mature primary mouse hippocampal neurons toward more mushroom-type and fewer thin-type spines [1]. ZEB85 (100 nM; 21 h) completely reverses Aβ 1-42 -induced dendritic spine loss and altered spine type distribution in mature primary mouse hippocampal neurons [1]. ZEB85 (100 nM; 15 min-2 h) activates the downstream TrkB signaling pathway in mature primary mouse hippocampal neurons and upregulates the expression of cFOS and pERK, whereas 10 nM ZEB85 fails to significantly activate this signaling pathway [1]. ZEB85 (100 nM; 24 h) increases the amplitude of spontaneous calcium transients, a marker of neuronal activity, in mature primary mouse hippocampal neurons [1]. ZEB85 (100 nM; 5-6 days) rescues the LTP induction deficit in hippocampal organotypic cultures of BDNF het KO mouse brain slices, increases the success rate of LTP induction to 60%, and promotes stable and long-lasting synaptic potentiation [1]. ZEB85 (100 nM; 5-30 min) activates the canonical TrkB signaling pathway (phosphorylation of PLCγ, AKT, and ERK) in CHO-NFAT human TrkB reporter cells, with a magnitude and time course of effect similar to that of BDNF [2]. ZEB85 potently induces TrkB phosphorylation in γ-aminobutyric acidergic neurons derived from H9 human embryonic stem cells, with an EC 50 of 470 pM [2]. ZEB85 (50 ug/mL; 15-240 min) activates the phosphorylation of TrkB in cortical neurons of embryonic day 21 mice [2]. ZEB85 (50 ug/mL; 3 days) significantly enhances dendritic growth of retinal ganglion cells in cultured adult mouse retinal explants [2].
Purity:
>95% purity as determined by SDS-PAGE.
Endotoxin Level:
< 10 EU/mg
Formulation:
0.01M PBS pH 7.4
State of Matter:
Liquid
Regulatory Status:
For research use only. Not suitable for clinical or therapeutic use.
Product Preparation:
Protein A/G purified from cell culture supernatant.
Storage and Handling:
Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
