Human Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2) ELISA Kit
The Human Tumor Necrosis Factor Alpha-Induced Protein 2 (TNFAIP2) ELISA Kit is specifically designed for the precise detection of TNFAIP2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for various research applications.TNFAIP2 is a key protein involved in the immune response and inflammation, particularly in response to tumor necrosis factor alpha stimulation. Its dysregulation has been linked to various diseases, such as cancer, autoimmune disorders, and inflammatory conditions.
As such, measuring TNFAIP2 levels can provide valuable insights into disease pathogenesis and potential therapeutic strategies. Overall, the Human TNFAIP2 ELISA Kit is a valuable tool for researchers looking to study the role of TNFAIP2 in various physiological and pathological processes, ultimately contributing to advancements in diagnosis and treatment of related diseases.
Product Name:
Human Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2) ELISA Kit
Product Code:
HUDL02931
Size:
96 Assays
Target:
TNFaIP2
Synonyms:
Primary response gene B94 protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
