Description
Human UBE2M Recombinant Protein (N-His)
Human UBE2M Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Lys183, with an N-terminal His tag. Predicted molecular weight: 23.06 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. NEDD8-conjugating enzyme Ubc12 (UBE2M) is a ~20 kDa protein. Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. For research use only.
The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
| Product Name: | Human UBE2M Recombinant Protein (N-His) |
| SKU: | GXRP01885 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M, UBE2M, UBC12, NEDD8-conjugating enzyme Ubc12 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Lys183 |
| Calculated MW: | 23.06 kDa |
| UniProt: | P61081 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
