UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. In order to degrade a protein, the ubiquitin-editing complex targets it for degradation via at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. It is known that the protein NEDD8, which can be conjugated to cellular proteins like Cdc53/culin, interacts with this. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1; however not RBX2; suggests that the RBX1-UBE2M complex neddylates particular target proteins, such as CUL1, CUL2, and CUL3. It is involved in cell proliferation and serves as a key cellular mechanism for the degradation of aberrant or short-lived proteins. Human UBE2M/UBC12 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human UBE2M/UBC12 Recombinant Protein (RPES3853)
Product Code:
RPES3853
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBC12, UBE2M,
Accession:
P61081
Sequence:
Met1-Lys183
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human UBE2M is produced by our E.coli expression system and the target gene encoding Met1-Lys183 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
20.9 kDa
AP Mol Mass:
25 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 50mM HEPES;pH 7.5;2mM DTT;150mM NaCl;10% Glycerol.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
