Human Ubiquitin Conjugating Enzyme E2A (UBE2A) ELISA Kit
The Human Ubiquitin-Conjugating Enzyme E2A (UBE2A) ELISA Kit is a powerful tool for the precise measurement of UBE2A levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for various research applications.UBE2A is a vital enzyme involved in the ubiquitination process, a key mechanism for protein degradation and regulation in cells. Dysregulation of UBE2A has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
By assessing UBE2A levels, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets.With the Human UBE2A ELISA Kit, researchers can delve deeper into the intricate world of ubiquitination and its role in human health and disease. This cutting-edge kit offers unparalleled precision and reliability, enabling researchers to advance their studies and make groundbreaking discoveries in the field of molecular biology.
Product Name:
Human Ubiquitin Conjugating Enzyme E2A (UBE2A) ELISA Kit
Product Code:
HUDL03031
Size:
96 Assays
Synonyms:
UBC2, HHR6A, RAD6 Homolog, Ubiquitin carrier protein A, Ubiquitin-protein ligase A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
