Human Uracil Phosphoribosyltransferase (UPRT) ELISA Kit
The Human UPRT (Uracil Phosphoribosyltransferase) ELISA Kit is specifically designed for the precise quantification of UPRT levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.UPRT is an important enzyme involved in the pyrimidine salvage pathway, playing a crucial role in the biosynthesis of nucleotides and DNA repair processes. Dysregulation of UPRT has been implicated in various diseases, including cancer, metabolic disorders, and neurological conditions, making it a valuable biomarker for understanding these diseases and developing potential therapeutic interventions.
The Human UPRT ELISA Kit from Assay Genie provides researchers with a reliable tool to accurately measure UPRT levels, facilitating in-depth investigations into the role of this enzyme in health and disease. This kit is user-friendly, cost-effective, and suitable for high-throughput analysis, making it an essential addition to any laboratory studying UPRT-related mechanisms.
Product Name:
Human Uracil Phosphoribosyltransferase (UPRT) ELISA Kit
Product Code:
HUDL03057
Size:
96 Assays
Target:
UPRT
Synonyms:
FUR1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
