Description
Human USP7 Recombinant Protein (N-His)
Human USP7 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Glu560-Asn1102, with an N-terminal His tag. Predicted molecular weight: 65.90 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ubiquitin carboxyl-terminal hydrolase 7 is a ~128 kDa protein. Hydrolase that deubiquitinates target proteins such as ARMC5, FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. For research use only.
Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity.
| Product Name: | Human USP7 Recombinant Protein (N-His) |
| SKU: | GXRP12215 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Deubiquitinating enzyme 7, EC:3.4.19.12, HAUSP, Herpesvirus-associated ubiquitin-specific protease, USP7, Ubiquitin carboxyl-terminal hydrolase 7, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Glu560-Asn1102 |
| Calculated MW: | 65.90 kDa |
| UniProt: | Q93009 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
