Ubiquitin carboxyl-terminal hydrolase 7 (Ubiquitin thioesterase 7/Herpesvirus-associated ubiquitin-specific protease/Ubiquitin-specific-processing protease 7/USP7/HAUSP) is a widely expressed protein which belongs to the peptidase C19 family. The USP7 enzyme is part of the deubiquitinating enzyme family. It controls stress response pathways, epigenetic silencing, and the proliferation of DNA virus infections. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. USP7 deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. TP53-dependent cell growth repression and apoptosis are also induced by USP7. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. ICP0, a regulatory protein of Herpes simplex virus type 1 (HSV-1), stimulates lytic infection and reactivation of latent viral genomes. ICP0 interacts very strongly with USP7. In productive HSV-1 infection, USP7-mediated preservation of ICP0 is more important than ICP0-induced loss of USP7. The biological significance of the ICP0-USP7 link may be most evident in natural infection scenarios, in which little quantities of ICP0 are produced. Human USP7/HAUSP Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human USP7/HAUSP Recombinant Protein (RPES0987)
Product Code:
RPES0987
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
HAUSP,TEF1
Accession:
NP_003461.2
Sequence:
Lys 208-Glu 560
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the amino acid sequence (Lys 208-Glu 560) of human USP7 (NP_003461.2) expressed and purified, with two additional aa (Gly & Pro) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
