Human V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) ELISA Kit
The Human V-set and Immunoglobulin Domain Containing Protein 2 (VSIG2) ELISA Kit is a powerful tool for the quantitative measurement of VSIG2 levels in human samples including serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate and reliable results, making it an excellent choice for a variety of research applications.VSIG2 is a protein that plays a key role in immune regulation and is involved in cell adhesion and signaling processes. Dysregulation of VSIG2 has been implicated in various diseases, including cancer and autoimmune disorders, making it an important biomarker for understanding disease mechanisms and developing targeted therapies.
With the Human VSIG2 ELISA Kit, researchers can accurately measure VSIG2 levels, opening up new possibilities for exploring its biological functions and potential clinical applications. Upgrade your research capabilities with this advanced ELISA kit designed for precise and efficient analysis of VSIG2 protein levels.
Product Name:
Human V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) ELISA Kit
Product Code:
HUDL03107
Size:
96 Assays
Target:
VSIG2
Synonyms:
CTXL, CTH, Cortical thymocyte-like protein
Detection Method:
Competition
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
