Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
The Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is specifically designed for the precise measurement of VEGF-A levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable tool for various research studies.VEGF-A, also known as vascular endothelial growth factor A, is a key protein involved in angiogenesis – the formation of new blood vessels from existing ones. It plays a crucial role in various physiological processes and pathological conditions, including cancer, cardiovascular diseases, and neurodegenerative disorders. Therefore, the detection and quantification of VEGF-A levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human VEGF-A ELISA Kit offers researchers a reliable and efficient method for studying the role of VEGF-A in health and disease, making it an essential tool for advancing scientific knowledge in the field of angiogenesis and related research areas.
Product Name:
Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
SKU:
HUES01397
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
The effect of hyperbaric oxygen therapy on hypospadias reconstruction: A preliminary randomized controlled trial study of VEGF levels and HOPE score analysis
