Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
The Assay Genie Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is a specialized assay crafted to quantitatively detect and measure VEGF-A levels in various biological samples obtained from mouse models. VEGF-A plays a key role in promoting angiogenesis and vasculogenesis, contributing significantly to the development of new blood vessels and endothelial growth in mouse tissues and organs. Our Mouse VEGF-A ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results essential for studying vascular development, angiogenesis, and related processes in mouse models. Manufactured under rigorous quality control standards, this kit promises robust performance and ease of use, making it an excellent choice for research focused on investigating VEGF-A's role in biological pathways and therapeutic strategies. Trust the Assay Genie Mouse VEGF-A ELISA Kit for accurate quantification of Vascular Endothelial Growth Factor A in your research endeavors.
Product Name:
Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
SKU:
AEES00191
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
