The Human VF (Visfatin) CLIA Kit is specifically designed for the quantitative determination of Visfatin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and accuracy, this kit provides researchers with reliable and reproducible results for a variety of research applications. Visfatin, also known as nicotinamide phosphoribosyltransferase (NAMPT), is a key protein involved in various physiological processes such as metabolism, inflammation, and insulin sensitivity.
Abnormal levels of Visfatin have been linked to conditions like obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for understanding these conditions and potentially developing targeted therapies. This Human VF CLIA Kit is a valuable tool for researchers looking to investigate the role of Visfatin in human health and disease, providing accurate and precise measurements for insightful research findings.
Product Name:
Human VF (Visfatin) CLIA Kit
SKU:
HUES00985
Target:
Human VF (Visfatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VF. You can calculate the concentration of Human VF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
97-111
98-116
Average (%)
108
105
106
1:4
Range (%)
102-115
84-99
89-101
Average (%)
108
91
95
1:8
Range (%)
93-108
100-116
91-105
Average (%)
99
106
98
1:16
Range (%)
96-106
96-111
85-99
Average (%)
101
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.85
44.62
180.11
23.3
43.3
177.99
Standard deviation
2.8
5.27
20.42
2.15
4.14
15.91
C V (%)
10.83
11.81
11.34
9.23
9.56
8.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
