The Human VIM (Vimentin) CLIA Kit is a cutting-edge assay designed for the accurate quantification of vimentin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research purposes.Vimentin is a key intermediate filament protein that plays a crucial role in maintaining cell structure and function. It is involved in processes such as cell migration, adhesion, and signaling, making it a critical biomarker for studying various diseases and conditions, including cancer, wound healing, and inflammation.
With the Human VIM (Vimentin) CLIA Kit, researchers can confidently measure vimentin levels in human samples, providing valuable insights into the role of this protein in health and disease. This kit is a valuable tool for advancing research in cell biology, cancer biology, and other fields, aiding in the development of new therapeutic strategies and interventions.
Product Name:
Human VIM (Vimentin) CLIA Kit
SKU:
HUES00642
Target:
Human VIM (Vimentin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VIM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VIM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VIM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VIM. You can calculate the concentration of Human VIM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
97-111
86-96
Average (%)
94
102
91
1:4
Range (%)
92-109
94-108
102-115
Average (%)
100
102
108
1:8
Range (%)
94-110
94-105
89-99
Average (%)
101
99
94
1:16
Range (%)
91-102
88-98
85-97
Average (%)
97
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
107
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
88-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
208.16
343.52
1564.42
189.76
341.33
1570.91
Standard deviation
18.55
28.17
171.15
17.15
37.61
118.92
C V (%)
8.91
8.2
10.94
9.04
11.02
7.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VIM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VIM in samples. No significant cross-reactivity or interference between Human VIM and analogues was observed.
