Human Wingless Type MMTV Integration Site Family, Member 9B (WNT9B) ELISA Kit
The Human Wingless-Type MMTV Integration Site Family Member 9B (WNT9B) ELISA Kit is specifically designed for the accurate detection of WNT9B levels in human serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.WNT9B is a key member of the WNT family of proteins, playing a critical role in cell signaling pathways and embryonic development. Dysregulation of WNT9B has been implicated in various diseases, including cancer and developmental disorders, highlighting its importance as a potential therapeutic target.
With the Human WNT9B ELISA Kit, researchers can effectively study the role of WNT9B in disease progression and explore potential therapeutic interventions. This kit provides a valuable tool for advancing scientific understanding and potentially developing new treatments for WNT9B-related conditions.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 9B (WNT9B) ELISA Kit
Product Code:
HUDL03135
Size:
96 Assays
Target:
WNT9B
Synonyms:
WNT15, WNT14B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
