Human WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit
The Human WISP2 (WNT1 inducible signaling pathway protein 2) ELISA Kit is designed for the precise measurement of WISP2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.WISP2 is a key protein in the WNT1 signaling pathway, playing a critical role in cell growth, differentiation, and tissue regeneration.
It is implicated in various diseases, including cancer, diabetes, and fibrosis, making it a valuable biomarker for studying these conditions and exploring potential treatment options.With its superior performance and ease of use, the Human WISP2 ELISA Kit is an essential tool for researchers investigating the role of WISP2 in health and disease. Order yours today from AssayGenie for reliable and high-quality results in your studies.
Product Name:
Human WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit
Product Code:
HUDL03121
Size:
96 Assays
Target:
WISP2
Synonyms:
CCN5, CT58, CTGF-L, CCN family member 5, Connective tissue growth factor-like protein, Connective tissue growth factor-related protein 58
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
