Human Xeroderma Pigmentosum, Complementation Group D (XPD) ELISA Kit
The Human Xeroderma Pigmentosum Complementation Group D (XPD) ELISA Kit is a powerful tool for the precise measurement of XPD levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for a variety of research purposes.XPD is a key protein involved in DNA repair mechanisms, particularly in the nucleotide excision repair pathway. Mutations in the XPD gene are associated with xeroderma pigmentosum, a rare genetic disorder characterized by extreme sensitivity to ultraviolet (UV) radiation and a high risk of skin cancer.
By measuring XPD levels, researchers can gain insights into DNA repair processes and their implications for human health.This ELISA kit enables scientists to explore the role of XPD in DNA damage response, cancer development, and other conditions linked to impaired DNA repair. Its reliability and precision make it an essential tool for advancing our understanding of XPD biology and developing targeted therapies for XPD-related disorders.
Product Name:
Human Xeroderma Pigmentosum, Complementation Group D (XPD) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
