The Human YY1 Transcription Factor (YY1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of YY1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and reproducibility, ensuring precise results for a variety of research applications.YY1 is a vital transcription factor that plays a crucial role in regulating gene expression and controlling multiple cellular processes. It is involved in diverse physiological functions such as cell proliferation, differentiation, and apoptosis.
Dysregulation of YY1 has been linked to various diseases including cancer, autoimmune disorders, and developmental abnormalities, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human YY1 Transcription Factor (YY1) ELISA Kit is an essential tool for researchers seeking to investigate the role of YY1 in disease pathogenesis and develop targeted therapies based on its modulation.
Product Name:
Human YY1 Transcription Factor (YY1) ELISA Kit
Product Code:
HUDL03154
Size:
96 Assays
Target:
YY1
Synonyms:
NF-E1, DELTA, UCRBP, YIN-YANG-1, INO80S, INO80 Complex Subunit S, Yin and Yang 1 Protein, Delta transcription factor, INO80 complex subunit S
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
