Transcriptional repressor protein YY1?YY1?contains 4 C2H2-type zinc fingers and belongs to the YY transcription factor family. Multifunctional transcription factor exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. The effect on transcription regulation of the protein is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression.
Product Name:
Human YY1 Recombinant Protein (RPES1210)
Product Code:
RPES1210
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Transcriptional repressor protein YY1,Delta transcription factor,INO80 complex subunit S,NF-E1,Yin and yang 1,INO80S
Accession:
P25490
Sequence:
Val221-Gly321
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Yin and Yang 1 protein/Transcriptional repressor protein YY1 is produced by our E.coli expression system and the target gene encoding Val221-Gly321 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
12.6 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
