The Iba1 Antibody (CAB1527) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human and mouse samples, making it suitable for use in various research applications such as immunofluorescence and immunohistochemistry.Iba1, also known as ionized calcium-binding adapter molecule 1, is a calcium-binding protein expressed in macrophages and microglia, playing a crucial role in the phagocytic activity and inflammatory response of these cells. Increased expression of Iba1 is often associated with neuroinflammation and neurodegenerative diseases, making it a promising target for research in areas such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Iba1 Antibody
SKU:
CAB1527
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain.
Purification Method
Affinity purification
Gene ID
199
RRID
AB_2762169
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AIF1/IBA1 Rabbit pAb (CAB1527) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using AIF1/IBA1 Rabbit pAb (CAB1527) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
