Anti-IGF2BP3 Antibody (CAB6099)

Antibody Name:	Anti-IGF2BP3 Antibody
Antibody SKU:	CAB6099
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, A-549, 293T, HeLa, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3 (NP_006538.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KKIE QDTD TKIT ISPL QELT LYNP ERTI TVKG NVET CAKA EEEI MKKI RESY ENDI ASMN LQAH LIPG LNLN ALGL FPPT SGMP PPTS GPPS AMTP PYPQ FEQS ETET VHLF IPAL SVGA IIGK QGQH IKQL SRFA GASI KIAP AEAP DAKV RMVI ITGP PEAQ FKAQ GRIY GKIK EENF VSPK EEVK LEAH IRVP SFAA GRVI GKGG KTVN ELQN LSSA EVVV PRDQ TPDE NDQV VVKI TGHF YACQ VAQR KIQE ILTQ VKQH QQQK ALQS GPPQ SRRK
Gene ID:	10643
Uniprot:	O00425
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	21kDa/63kDa
Observed MW:	70kDa

Synonyms:

IGF2BP3, CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3

Background:

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.

UniProt Protein Function:	IGF2BP3: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. Belongs to the RRM IMP/VICKZ family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Spliceosome; Translation; RNA-binding Chromosomal Location of Human Ortholog: 7p11 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding Biological Process: anatomical structure morphogenesis; mRNA transport; translation; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process
NCBI Summary:	The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]
UniProt Code:	O00425
NCBI GenInfo Identifier:	254763311
NCBI Gene ID:	10643
NCBI Accession:	O00425.2
UniProt Secondary Accession:	O00425,Q63HM0, Q6MZZ2, Q86VB1, A0A4Z5,
UniProt Related Accession:	O00425
Molecular Weight:	579
NCBI Full Name:	Insulin-like growth factor 2 mRNA-binding protein 3
NCBI Synonym Full Names:	insulin-like growth factor 2 mRNA binding protein 3
NCBI Official Symbol:	IGF2BP3
NCBI Official Synonym Symbols:	KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
NCBI Protein Information:	insulin-like growth factor 2 mRNA-binding protein 3; VICKZ family member 3; cancer/testis antigen 98; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; KH domain containing protein overexpressed in cancer
UniProt Protein Name:	Insulin-like growth factor 2 mRNA-binding protein 3
UniProt Synonym Protein Names:	IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer; hKOC; VICKZ family member 3
Protein Family:	Insulin-like growth factor 2 mRNA-binding protein
UniProt Gene Name:	IGF2BP3
UniProt Entry Name:	IF2B3_HUMAN

	Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (CAB6099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat testis using IGF2BP3 antibody (CAB6099) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using IGF2BP3 antibody (CAB6099) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using IGF2BP3 antibody (CAB6099) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using IGF2BP3 antibody (CAB6099) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-IGF2BP3 Antibody (CAB2750)	Human IMP3 ELISA Kit
Anti-IGF2BP3 Antibody (CAB4444)	Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) CLIA Kit (HUES00315)
IGF2BP3 Antibody (PACO09893)	Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit (HUES01650)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)