null

Anti-IKKEpsilon Antibody (CAB14093)

SKU:
CAB14093
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-IKKEpsilon Antibody CAB14093
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-IKKEpsilon Antibody CAB14093
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-IKKEpsilon Antibody CAB14093
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-IKKEpsilon Antibody CAB14093
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-IKKEpsilon Antibody CAB14093
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:IKK? Rabbit Polyclonal Antibody
Antibody SKU:CAB14093
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 495-716 of human IKK? (NP_054721.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, 293T, SW480, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 495-716 of human IKK? (NP_054721.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LRSR LRTL AEVL SRCS QNIT ETQE SLSS LNRE LVKS RDQV HEDR SIQQ IQCC LDKM NFIY KQFK KSRM RPGL GYNE EQIH KLDK VNFS HLAK RLLQ VFQE ECVQ KYQA SLVT HGKR MRVV HETR NHLR LVGC SVAA CNTE AQGV QESL SKLL EELS HQLL QDRA KGAQ ASPP PIAP YPSP TRKD LLLH MQEL CEGM KLLA SDLL DNNR IIER LNRV PAPP DV
Gene ID:9641
Uniprot:Q14164
Cellular Location:Cytoplasm, Nucleus, PML body
Calculated MW:70kDa/80kDa
Observed MW:80kDa
Synonyms:IKBKE, IKK-E, IKK-i, IKKE, IKKI
Background:
UniProt Protein Function:IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel.
UniProt Protein Details:

Protein type:Inhibitor; Kinase, protein; EC 2.7.11.10; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; PML body; cytoplasm; mitochondrial membrane; endosome membrane; nucleus; cytosol

Molecular Function:NF-kappaB-inducing kinase activity; protein binding; IkappaB kinase activity; ATP binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; DNA damage response, signal transduction resulting in induction of apoptosis; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; toll-like receptor 3 signaling pathway; I-kappaB phosphorylation; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; protein amino acid phosphorylation

NCBI Summary:IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]
UniProt Code:Q14164
NCBI GenInfo Identifier:14548079
NCBI Gene ID:9641
NCBI Accession:Q14164.1
UniProt Secondary Accession:Q14164,Q3B754, Q3KR43, Q5JTS6, D3DT78,
UniProt Related Accession:Q14164
Molecular Weight:716
NCBI Full Name:Inhibitor of nuclear factor kappa-B kinase subunit epsilon
NCBI Synonym Full Names:inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
NCBI Official Symbol:IKBKE  
NCBI Official Synonym Symbols:IKKE; IKKI; IKK-E; IKK-i  
NCBI Protein Information:inhibitor of nuclear factor kappa-B kinase subunit epsilon; IKK-epsilon; I-kappa-B kinase epsilon; inducible IkappaB kinase; IKK-related kinase epsilon; inducible I kappa-B kinase
UniProt Protein Name:Inhibitor of nuclear factor kappa-B kinase subunit epsilon
UniProt Synonym Protein Names:Inducible I kappa-B kinase; IKK-i
UniProt Gene Name:IKBKE  
UniProt Entry Name:IKKE_HUMAN
Anti-IKKEpsilon Antibody (CAB14093)Western blot analysis of extracts of various cell lines, using IKKe antibody (CAB14093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-IKKEpsilon Antibody (CAB14093)Immunohistochemistry of paraffin-embedded rat spleen using IKKe antibody (CAB14093) at dilution of 1:100 (40x lens).
Anti-IKKEpsilon Antibody (CAB14093)Immunohistochemistry of paraffin-embedded mouse brain using IKKe antibody (CAB14093) at dilution of 1:100 (40x lens).
Anti-IKKEpsilon Antibody (CAB14093)Immunofluorescence analysis of C6 cells using IKKe antibody (CAB14093) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-IKKEpsilon Antibody (CAB14093)Immunofluorescence analysis of HeLa cells using IKKe antibody (CAB14093) at dilution of 1:100. Blue: DAPI for nuclear staining.

Related Products