The IL12 Monoclonal Antibody (CAB23157) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed for use in various research applications, is highly specific for detecting IL12 and is validated for use in techniques such as ELISA and flow cytometry.Interleukin-12 is a key player in the immune system, influencing the differentiation and activation of T-cells and natural killer cells. By specifically targeting IL12, researchers can gain valuable insights into immune system function, inflammation, and potential therapeutic targets for diseases such as cancer and autoimmune disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IL12 Monoclonal Antibody
SKU:
CAB23157
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC60328
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Purification Method
Affinity purification
Gene ID
3593
RRID
AB_3678805
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T-IL12-His(N), using IL12 Rabbit mAb (CAB23157) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
