The IL1A Antibody (CAB1316) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards IL1A in human samples, making it suitable for use in Western blot applications. By targeting the IL1A protein, this antibody enables precise detection and analysis in a variety of cell types, providing researchers with crucial insights into the role of IL1A in immunology and disease pathogenesis.IL1A, also known as interleukin-1 alpha, is a key mediator of inflammation and immune regulation, playing a critical role in the body's defense against infections and injury. Dysregulation of IL1A has been implicated in the pathogenesis of various diseases, including autoimmune disorders, cardiovascular diseases, and cancer.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IL1A Antibody
SKU:
CAB1316
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL1, IL-1A, IL1F1, IL1-ALPHA, IL-1 alpha
Positive Sample:
Rat lung, Recombinant Human IL-1 alpha Protein
Cellular Localization:
Secreted.
Calculated MW:
31kDa
Observed MW:
31kDa/21kDa
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
3552
RRID
AB_2760010
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using IL-1 alpha Rabbit pAb (CAB1316) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of Recombinant Human IL-1 alpha Protein (RP00098), using IL-1 alpha Rabbit pAb (CAB1316) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using IL-1 alpha Rabbit pAb (CAB1316) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
