Western blot analysis of Recombinant Human IL-2 Protein(1ng), using IL2 Rabbit mAb (CAB23990) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The IL2 Monoclonal Antibody (CAB23990) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using monoclonal antibody technology, is specifically designed for use in various immunological research applications, including Western blot and immunohistochemistry.IL-2 is known for its important functions in promoting the growth and activation of T cells, which are key players in the immune response against infections and cancer. By targeting IL-2, researchers can gain insights into how this cytokine contributes to immune system function and dysfunction, offering potential avenues for the development of immunotherapies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IL2 Monoclonal Antibody
SKU:
CAB23990
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC55030
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL-2, TCGF, lymphokine, IL2
Positive Sample:
Recombinant Human IL-2 Protein, Jurkat treated with PMA, A23187 and BFA
Cellular Localization:
Secreted.
Calculated MW:
17kDa
Observed MW:
16kDa/18kDa/14kDa
This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Purification Method
Affinity purification
Gene ID
3558
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of Recombinant Human IL2 Protein (RP01039), using IL2 Rabbit mAb (CAB23990) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Jurkat cells using IL2 Rabbit mAb (CAB23990) at 1:7000 dilution incubated at room temperature for 1.5 hours. Jurkat cells were treated with PMA (40 nM), A23187 (2 μM) and BFA (300 ng/mL) for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20 s.
