The IL31 Antibody (CAB14982) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for detecting IL-31 in human samples and has been validated for use in Western blot applications.IL-31 is known for its role in promoting inflammation and itching in conditions such as atopic dermatitis and allergic diseases. By targeting IL-31 with this polyclonal antibody, researchers can gain insights into the signaling pathways and mechanisms regulated by this cytokine, aiding in the development of new therapies for inflammatory and allergic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IL31 Antibody
SKU:
CAB14982
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL-31, IL31
Positive Sample:
293T, HT-29, Mouse spleen, Mouse kidney, Mouse thymus, Rat testis
Cellular Localization:
Secreted.
Calculated MW:
18kDa
Observed MW:
18kDa
IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).
Purification Method
Affinity purification
Gene ID
386653
RRID
AB_2761866
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IL31 Rabbit pAb (CAB14982) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
