Anti-ILF2 Antibody (CAB5882)

Antibody Name:	Anti-ILF2 Antibody
Antibody SKU:	CAB5882
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ILF2 (NP_004506.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Raji, A-431, HeLa, Mouse brain, Mouse spleen, Mouse ovary, Mouse thymus, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ILF2 (NP_004506.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRGD RGRG RGGR FGSR GGPG GGFR PFVP HIPF DFYL CEMA FPRV KPAP DETS FSEA LLKR NQDL APNS AEQA SILS LVTK INNV IDNL IVAP GTFE VQIE EVRQ VGSY KKGT MTTG HNVA DLVV ILKI LPTL EAVA ALGN KVVE SLRA QDPS EVLT MLTN ETGF EISS SDAT VKIL ITTV PPNL RKLD PELH LDIK VLQS ALAA IRHA RWFE ENAS QSTV KVLI RLLK DLRI RFPG FEPL TPWI LDLL GHYA VMNN PTRQ PLAL NVAY RRCL QILA AGLF
Gene ID:	3608
Uniprot:	Q12905
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	43kDa
Observed MW:	50kDa

Synonyms:

ILF2, NF45, PRO3063

Background:

The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.

UniProt Protein Function:	ILF2: Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
UniProt Protein Details:	Protein type:RNA-binding; Transcription, coactivator/corepressor; Nucleolus Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus Molecular Function:transferase activity; protein binding; DNA binding; double-stranded RNA binding; ATP binding Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; immune response
NCBI Summary:	The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]
UniProt Code:	Q12905
NCBI GenInfo Identifier:	62510764
NCBI Gene ID:	3608
NCBI Accession:	Q12905.2
UniProt Secondary Accession:	Q12905,Q5SR10, Q5SR11, Q7L7R3, Q9BWD4, Q9P1N0, A6NDB0 B2R8G7,
UniProt Related Accession:	Q12905
Molecular Weight:	390
NCBI Full Name:	Interleukin enhancer-binding factor 2
NCBI Synonym Full Names:	interleukin enhancer binding factor 2
NCBI Official Symbol:	ILF2
NCBI Official Synonym Symbols:	NF45; PRO3063
NCBI Protein Information:	interleukin enhancer-binding factor 2; interleukin enhancer binding factor 2, 45kD; nuclear factor of activated T-cells, 45-kDa; interleukin enhancer binding factor 2, 45kDa
UniProt Protein Name:	Interleukin enhancer-binding factor 2
UniProt Synonym Protein Names:	Nuclear factor of activated T-cells 45 kDa
Protein Family:	Interleukin enhancer-binding factor
UniProt Gene Name:	ILF2
UniProt Entry Name:	ILF2_HUMAN

	Western blot analysis of extracts of various cell lines, using ILF2 antibody (CAB5882) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 300s.
	Immunohistochemistry of paraffin-embedded rat brain using ILF2 Antibody (CAB5882) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using ILF2 antibody (CAB5882).

Antibodies

Anti-ILF2 Antibody (CAB13320)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)