Anti-IMPDH1 Antibody (CAB9497)

Antibody Name:	Anti-IMPDH1 Antibody
Antibody SKU:	CAB9497
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 434-563 of human IMPDH1 (NP_899066.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NIH/3T3, 293T, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 434-563 of human IMPDH1 (NP_899066.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MMGS LLAA TTEA PGEY FFSD GVRL KKYR GMGS LDAM EKSS SSQK RYFS EGDK VKIA QGVS GSIQ DKGS IQKF VPYL IAGI QHGC QDIG ARSL SVLR SMMY SGEL KFEK RTMS AQIE GGVH GLHS YEKR LY
Gene ID:	3614
Uniprot:	P20839
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	52kDa/54kDa/55kDa/60kDa/63kDa/64kDa
Observed MW:	55KDa

Synonyms:

IMPDH1, IMPD, IMPD1, IMPDH-I, LCA11, RP10, sWSS2608

Background:

The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	IMPDH1: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant. Defects in IMPDH1 are the cause of Leber congenital amaurosis type 11 (LCA11). LCA11 is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the IMPDH/GMPR family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine; EC 1.1.1.205 Chromosomal Location of Human Ortholog: 7q31.3-q32 Cellular Component: cell junction; cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:DNA binding; IMP dehydrogenase activity; nucleic acid binding Biological Process: purine ribonucleoside monophosphate biosynthetic process Disease: Leber Congenital Amaurosis 11; Retinitis Pigmentosa 10
NCBI Summary:	The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
UniProt Code:	P20839
NCBI GenInfo Identifier:	25014074
NCBI Gene ID:	3614
NCBI Accession:	P20839.2
UniProt Secondary Accession:	P20839,Q8N194, Q96NU2, A4D0Z6, A4D0Z7, A6NDW5, A6NNI6 B3KNP7, B3KVM8, B4DE09, C9JV30, J3KNX8,
UniProt Related Accession:	P20839
Molecular Weight:	60,898 Da
NCBI Full Name:	Inosine-5'-monophosphate dehydrogenase 1
NCBI Synonym Full Names:	inosine monophosphate dehydrogenase 1
NCBI Official Symbol:	IMPDH1
NCBI Official Synonym Symbols:	IMPD; RP10; IMPD1; LCA11; IMPDH-I; sWSS2608
NCBI Protein Information:	inosine-5'-monophosphate dehydrogenase 1
UniProt Protein Name:	Inosine-5'-monophosphate dehydrogenase 1
UniProt Synonym Protein Names:	IMPDH-I
Protein Family:	Inosine-5'-monophosphate dehydrogenase
UniProt Gene Name:	IMPDH1
UniProt Entry Name:	IMDH1_HUMAN

	Western blot analysis of extracts of various cell lines, using IMPDH1 Rabbit pAb (CAB9497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using IMPDH1 Rabbit pAb (CAB9497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-IMPDH1 Antibody (CAB16899)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)