Anti-InaD-like protein Antibody (CAB8476)

Antibody Name:	Anti-InaD-like protein Antibody
Antibody SKU:	CAB8476
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 450-680 of human INADL (NP_795352.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	SKOV3, SW480, 293T, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 450-680 of human INADL (NP_795352.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VRRK TSSS TSPL EPPS DRGT VVEP LKPP ALFL TGAV ETET NVDG EDEE IKER IDTL KNDN IQAL EKLE KVPD SPEN ELKS RWEN LLGP DYEV MVAT LDTQ IADD AELQ KYSK LLPI HTLR LGVE VDSF DGHH YISS IVSG GPVD TLGL LQPE DELL EVNG MQLY GKSR REAV SFLK EVPP PFTL VCCR RLFD DEAS VDEP RRTE TSLP ETEV DHNM DVNT EEDD DGEL ALW
Gene ID:	10207
Uniprot:	Q8NI35
Cellular Location:	Apical cell membrane, Cell junction, Cell membrane, Cytoplasm, Peripheral membrane protein, perinuclear region, tight junction
Calculated MW:	125kDa/167kDa/170kDa/173kDa/196kDa
Observed MW:	255kDa

Synonyms:

PATJ, Cipp, INADL, InaD-like, hINADL

Background:

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.

UniProt Protein Function:	Scaffolding protein that may bring different proteins into adjacent positions at the cell membrane. May regulate protein targeting, cell polarity and integrity of tight junctions. May regulate the surface expression and/or function of ASIC3 in sensory neurons. May recruit ARHGEF18 to apical cell-cell boundaries (PubMed:22006950).
NCBI Summary:	This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8NI35
NCBI GenInfo Identifier:	116242542
NCBI Gene ID:	10207
NCBI Accession:	Q8NI35.3
UniProt Secondary Accession:	Q8NI35,O15249, O43742, O60833, Q5VUA5, Q5VUA6, Q5VUA7 Q5VUA8, Q5VUA9, Q5VUB0, Q8WU78, Q9H3N9,
UniProt Related Accession:	Q8NI35
Molecular Weight:	125,231 Da
NCBI Full Name:	InaD-like protein
NCBI Synonym Full Names:	PATJ, crumbs cell polarity complex component
NCBI Official Symbol:	PATJ
NCBI Official Synonym Symbols:	Cipp; INADL; hINADL; InaD-like
NCBI Protein Information:	inaD-like protein
UniProt Protein Name:	InaD-like protein
UniProt Synonym Protein Names:	Pals1-associated tight junction protein; Protein associated to tight junctions
Protein Family:	Patj
UniProt Gene Name:	PATJ

	Western blot analysis of extracts of various cell lines, using INADL antibody (CAB8476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse kidney using INADL antibody (CAB8476) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using INADL antibody (CAB8476) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using INADL antibody (CAB8476) at dilution of 1:100 (40x lens).

Antibodies

Anti-INADL Antibody (CAB12063)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)