The Integrin beta 3 (ITGB3/CD61) Monoclonal Antibody (CAB19073) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, offers high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the Integrin Beta 3 protein, allowing for detection and analysis in various cell types.Integrin Beta 3 is known for its role in cell adhesion and migration, making it an important target for studies in cell biology, cancer research, and angiogenesis.
This antibody is validated for use in WB, IP, ELISA, FC (intra) applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Integrin beta 3 (ITGB3/CD61) Monoclonal Antibody
SKU:
CAB19073
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0460
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Cell Junction, Cell Membrane, Cell Projection, Single-Pass Type I Membrane Protein, Focal Adhesion, Lamellipodium Membrane.
Calculated MW:
87kDa
Observed MW:
100kDa
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Purification Method
Affinity purification
Gene ID
3690
RRID
AB_2862565
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Integrin beta 3 (ITGB3/CD61) Rabbit mAb (CAB19073) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 300 μg extracts of U-87MG cells using 3 μg Integrin beta 3 (ITGB3/CD61) antibody (CAB19073). Western blot was performed from the immunoprecipitate using Integrin beta 3 (ITGB3/CD61) antibody (CAB19073) at a dilution of 1:1000.
