The ITGB5 Antibody (CAB2497) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the integrin beta 5 protein, this antibody allows for the detection and analysis of integrin beta 5 expression in various cell types, making it ideal for studies in cell biology and cancer research.Integrin beta 5 is known to play a crucial role in cell migration, invasion, and angiogenesis, making it a key player in tumor progression and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ITGB5 Antibody
SKU:
CAB2497
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ITGB5
Positive Sample:
A549
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
88kDa
Observed MW:
100kDa
This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.
Purification Method
Affinity purification
Gene ID
3693
RRID
AB_2764388
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A549 cells, using ITGB5 Rabbit pAb (CAB2497) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
