Anti-IFNG Antibody (CAB0242)

Antibody Name:	Anti-IFNG Antibody
Antibody SKU:	CAB0242
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFNG (NP_000610.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	BT-474, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFNG (NP_000610.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QDPY VKEA ENLK KYFN AGHS DVAD NGTL FLGI LKNW KEES DRKI MQSQ IVSF YFKL FKNF KDDQ SIQK SVET IKED MNVK FFNS NKKK RDDF EKLT NYSV TDLN VQRK AIHE LIQV MAEL SPAA KTGK RKRS QMLF RGRR ASQ
Gene ID:	3458
Uniprot:	P01579
Cellular Location:	Secreted
Calculated MW:	19kDa
Observed MW:	24kDa

Synonyms:

IFNG, IFG, IFI

Background:

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.

UniProt Protein Function:	IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Secreted; Secreted, signal peptide; Cytokine Chromosomal Location of Human Ortholog: 12q14 Cellular Component: extracellular space; extracellular region; external side of plasma membrane Molecular Function:interferon-gamma receptor binding; cytokine activity Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; apoptosis; positive regulation of interleukin-23 production; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; positive regulation of osteoclast differentiation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of chemokine biosynthetic process; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Tuberous Sclerosis 2; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
NCBI Summary:	This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]
UniProt Code:	P01579
NCBI GenInfo Identifier:	124479
NCBI Gene ID:	3458
NCBI Accession:	P01579.1
UniProt Secondary Accession:	P01579,Q53ZV4, B5BU88,
UniProt Related Accession:	P01579
Molecular Weight:
NCBI Full Name:	Interferon gamma
NCBI Synonym Full Names:	interferon, gamma
NCBI Official Symbol:	IFNG
NCBI Official Synonym Symbols:	IFG; IFI
NCBI Protein Information:	interferon gamma; IFN-gamma; immune interferon
UniProt Protein Name:	Interferon gamma
UniProt Synonym Protein Names:	Immune interferon
Protein Family:	Interferon
UniProt Gene Name:	IFNG
UniProt Entry Name:	IFNG_HUMAN

	Western blot analysis of extracts of various cell lines, using IFNG antibody (CAB0242) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human prostate using IFNG antibody (CAB0242) (40x lens).

Antibodies	ELISA Kits
AF/LE Purified Human IFN-gamma Monoclonal Antibody [B27](AGEL2023)	Human IFN-gamma (Interferon Gamma) CLIA Kit (HUES00104)
Human IFN-gamma Monoclonal Antibody (FITC Conjugated) [B27](AGEL2024)	Human IFN-gamma (Interferon Gamma) ELISA Kit (HUES01394)
Human IFN-gamma Monoclonal Antibody (PE Conjugated) [B27](AGEL2025)	Human Interferon gamma (IFNG) ELISA Kit
Human IFN-gamma Monoclonal Antibody (APC Conjugated) [B27](AGEL2026)
Anti-IFNG Antibody (CAB12450)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)