Anti-Interferon lambda-1 Antibody (CAB8288)

Antibody Name:	IFNL1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8288
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human IFNL1 (NP_742152.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human IFNL1 (NP_742152.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GPVP TSKP TTTG KGCH IGRF KSLS PQEL ASFK KARD ALEE SLKL KNWS CSSP VFPG NWDL RLLQ VRER PVAL EAEL ALTL KVLE AAAG PALE DVLD QPLH TLHH ILSQ LQAC IQPQ PTAG PRPR GRLH HWLH RLQE APKK ESAG CLEA SVTF NLFR LLTR DLKY VADG NLCL RTST HPES T
Gene ID:	282618
Uniprot:	Q8IU54
Cellular Location:	Secreted
Calculated MW:	21kDa
Observed MW:	22kDa

Synonyms:

IFNL1, IL-29, IL29

Background:

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).

UniProt Protein Function:	IL29: Cytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.
UniProt Protein Details:	Protein type:Cytokine; Secreted, signal peptide; Secreted; Cell cycle regulation Chromosomal Location of Human Ortholog: 19q13.13 Cellular Component: extracellular space; extracellular region; interleukin-28 receptor complex Molecular Function:cytokine activity; interleukin-28 receptor binding; receptor binding Biological Process: positive regulation of immune response; positive regulation of transcription, DNA-dependent; positive regulation of JAK-STAT cascade; JAK-STAT cascade; negative regulation of T cell differentiation; negative regulation of cell proliferation; positive regulation of interferon-gamma production; negative regulation of interleukin-13 production; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of T-helper 2 type immune response; negative regulation of interleukin-5 production; positive regulation of MHC class I biosynthetic process; negative regulation of transcription, DNA-dependent; negative regulation of memory T cell differentiation; defense response to virus
NCBI Summary:	This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]
UniProt Code:	Q8IU54
NCBI GenInfo Identifier:	26024325
NCBI Gene ID:	282618
NCBI Accession:	NP_742152
UniProt Secondary Accession:	Q8IU54,Q17R34, A0AV25,
UniProt Related Accession:	Q8IU54
Molecular Weight:	22.7kDa (206aa)
NCBI Full Name:	interferon lambda-1
NCBI Synonym Full Names:	interferon lambda 1
NCBI Official Symbol:	IFNL1
NCBI Official Synonym Symbols:	IL29; IL-29
NCBI Protein Information:	interferon lambda-1
UniProt Protein Name:	Interferon lambda-1
UniProt Synonym Protein Names:	Cytokine Zcyto21; Interleukin-29
UniProt Gene Name:	IFNL1
UniProt Entry Name:	IFNL1_HUMAN

	Western blot analysis of extracts of various cell lines, using IFNL1 antibody (CAB8288) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded human stomach using IFNL1 antibody (CAB8288) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
IFNL1 Antibody (PACO01998)	Human IL-29 ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)