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Western blot - Human IL-5 Rabbit pAb Western blot analysis of extracts of Recombinant Human IL-5 Protein, using Human IL-5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.
Key Features

Human IL-5 Polyclonal Antibody (CAB21170)

Datasheet MSDS
SKU CAB21170
Product Type Antibody
Antibody Type Polyclonal Antibody
Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Host Species Rabbit
Synonyms EDF, TRF, IL-5, Human IL-5
€96 - €370

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Description

system_update_altDatasheetsystem_update_altMSDS

Human IL-5 Polyclonal Antibody (CAB21170)

The Human IL-5 Polyclonal Antibody (CAB21170) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, exhibits high reactivity with human samples and is validated for use in Western blot assays.IL-5 plays a crucial role in promoting the growth and activation of eosinophils, a type of white blood cell involved in allergic reactions and asthma pathophysiology. By targeting IL-5, researchers can better understand its role in immune regulation and potentially develop novel therapeutic approaches for conditions characterized by eosinophilic inflammation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.

Product Name:Human IL-5 Polyclonal Antibody
SKU:CAB21170
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:IPTE IPTS ALVK ETLA LLST HRTL LIAN ETLR IPVP VHKN HQLC TEEI FQGI GTLE SQTV QGGT VERL FKNL SLIK KYID GQKK KCGE ERRR VNQF LDYL QEFL GVMN TEWI IES
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EDF, TRF, IL-5, Human IL-5
Positive Sample:Recombinant Human IL-5 Protein
Cellular Localization:Secreted.
Calculated MW:15kDa
Observed MW:18-23kDa

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

Purification MethodAffinity purification
Gene ID3567
RRIDIF
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of Recombinant Human IL-5 Protein (RP01316), using Human IL-5 Rabbit pAb (CAB21170) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.8s.
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