The IRF4 Antibody (CAB0524) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it suitable for use in Western blot applications. By binding to the IRF4 protein, it enables the detection and analysis of IRF4 in a variety of cell types.IRF4 is a transcription factor that plays a crucial role in the regulation of immune responses, particularly in the differentiation and function of immune cells. It is involved in a wide range of biological processes, including inflammation, immune cell development, and the immune response to pathogens.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IRF4 Antibody
SKU:
CAB0524
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MUM1, LSIRF, SHEP8, NF-EM5, IRF4
Positive Sample:
THP-1, Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
52kDa
Observed MW:
55kDa
The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
3662
RRID
AB_2757242
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IRF4 Rabbit pAb (CAB0524) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
