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Anti-IRF5 Antibody (CAB1149)

SKU:
CAB1149
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 1 Anti-IRF5 Antibody CAB1149
  • Immunology Antibodies 1 Anti-IRF5 Antibody CAB1149
€139 - €419
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Description

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Antibody Name:Anti-IRF5 Antibody
Antibody SKU:CAB1149
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Rat
Positive Samples:THP-1, U-937, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNQS IPVA PTPP RRVR LKPW LVAQ VNSC QYPG LQWV NGEK KLFC IPWR HATR HGPS QDGD NTIF KAWA KETG KYTE GVDE ADPA KWKA NLRC ALNK SRDF RLIY DGPR DMPP QPYK IYEV CSNG PAPT DSQP PEDY SFGA GEEE EEEE ELQR MLPS LSLT EDVK WPPT LQPP TLRP PTLQ PPTL QPPV VLGP PAPD PSPL
Gene ID:3663
Uniprot:Q13568
Cellular Location:Cytoplasm, Nucleus
Calculated MW:17kDa/47kDa/54kDa/56kDa/57kDa
Observed MW:56kDa/60kDa
Synonyms:IRF5, SLEB10
Background:This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
UniProt Protein Function:IRF5: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. Homodimer, when phosphorylated. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function:protein binding; transcription factor activity

Biological Process: transcription, DNA-dependent; positive regulation of apoptosis; response to muramyl dipeptide; positive regulation of interleukin-12 production; cytokine and chemokine mediated signaling pathway; response to peptidoglycan; positive regulation of interferon-beta production; positive regulation of transcription from RNA polymerase II promoter; defense response to virus; positive regulation of interferon-alpha production

Disease: Systemic Lupus Erythematosus, Susceptibility To, 10; Inflammatory Bowel Disease 14

NCBI Summary:This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]
UniProt Code:Q13568
NCBI GenInfo Identifier:20178305
NCBI Gene ID:3663
NCBI Accession:Q13568.2
UniProt Secondary Accession:Q13568,Q1A7B4, Q64GA9, Q64GB1, Q64GB2, Q6RCM8, Q9BQF0 A4D1J8, A8DUA8, A8DUA9, E7EQ16, E7EW54,
UniProt Related Accession:Q13568
Molecular Weight:498
NCBI Full Name:Interferon regulatory factor 5
NCBI Synonym Full Names:interferon regulatory factor 5
NCBI Official Symbol:IRF5  
NCBI Official Synonym Symbols:SLEB10  
NCBI Protein Information:interferon regulatory factor 5
UniProt Protein Name:Interferon regulatory factor 5
Protein Family:Interferon regulatory factor
UniProt Gene Name:IRF5  
UniProt Entry Name:IRF5_HUMAN
Anti-IRF5 Antibody (CAB1149)Western blot analysis of extracts of various cell lines, using IRF5 antibody (CAB1149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-IRF5 Antibody (CAB1149)Immunofluorescence analysis of HeLa cells using IRF5 antibody (CAB1149). Blue: DAPI for nuclear staining.

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