The ITGA10 Polyclonal Antibody (CAB16704) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it a valuable tool for studying ITGA10 expression and function.Integrins are essential for cell adhesion to the extracellular matrix and are involved in various physiological processes such as cell migration, proliferation, and differentiation. ITGA10, a member of the integrin alpha subunit family, is particularly important in mediating interactions between cells and the extracellular matrix in connective tissues.The ITGA10 Polyclonal Antibody enables researchers to detect and analyze ITGA10 protein levels in various cell types and tissues, providing valuable insights into its function and involvement in processes such as tissue development, wound healing, and fibrosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ITGA10 Polyclonal Antibody
SKU:
CAB16704
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PRO827, ITGA10
Positive Sample:
HUVEC
Cellular Localization:
External Side Of Plasma Membrane, Plasma Membrane.
Calculated MW:
128kDa
Observed MW:
128kDa
Integrins are integral transmembrane glycoproteins composed of noncovalently linked alpha and beta chains. They participate in cell adhesion as well as cell-surface mediated signalling. This gene encodes an integrin alpha chain and is expressed at high levels in chondrocytes, where it is transcriptionally regulated by AP-2epsilon and Ets-1. The protein encoded by this gene binds to collagen. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8515
RRID
AB_2770004
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HUVEC cells, using ITGA10 Rabbit pAb (CAB16704) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.8s.
