The ITSN2 Antibody (CAB16525) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ITSN2 protein, this antibody allows for the detection and analysis of ITSN2 in a variety of cell types, making it ideal for research in cell biology, neuroscience, and cancer biology.ITSN2 is known to play a crucial role in controlling cell proliferation, migration, and differentiation, making it a key player in multiple cellular processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ITSN2 Antibody
SKU:
CAB16525
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SWA, SWAP, SH3D1B, SH3P18, PRO2015, ITSN2
Positive Sample:
U-87MG, LO2, Jurkat, HeLa
Cellular Localization:
Cytoplasm.
Calculated MW:
193kDa
Observed MW:
180kDa
This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis.
Purification Method
Affinity purification
Gene ID
50618
RRID
AB_2770020
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ITSN2 Rabbit pAb (CAB16525) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using ITSN2 Rabbit pAb (CAB16525) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ITSN2 Rabbit pAb (CAB16525) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
