The JunD Monoclonal Antibody (CAB5433) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications.The JUND protein is known for its role in various biological processes, including cell growth, differentiation, and apoptosis. It is also implicated in diseases such as cancer, where its dysregulation can promote tumor growth and metastasis. By targeting the JUND protein, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.
This antibody is validated for use in WB, ChIP, ELISA, CUT&Tag applications and has demonstrated reactivity against Human samples.
Product Name:
JunD Monoclonal Antibody
SKU:
CAB5433
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1408
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AP-1, JunD
Positive Sample:
HeLa, HT-1080
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
38kDa/42kDa
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216).
Purification Method
Affinity purification
Gene ID
3727
RRID
AB_2863501
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using JunD Rabbit mAb (CAB5433) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using JunD antibody (CAB5433) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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