Western blot analysis of HepG2, using MAD1/MAD1L1 Rabbit pAb antibody (CAB22000) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] MAD1/MAD1L1 Polyclonal Antibody (CAB22000) is a high-quality antibody developed for reliable detection and analysis of target proteins. This pathway plays a critical role in ensuring accurate chromosome segregation during cell division, making it a key target for studying cell cycle regulation and genomic stability.Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications to detect and analyze the expression of MAD1 and MAD1L1 proteins in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KD Validated] MAD1/MAD1L1 Polyclonal Antibody
SKU:
CAB22000
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
8379
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using [KD Validated] MAD1/MAD1L1 Rabbit pAb (CAB22000) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type(WT) and MAD1/MAD1L1 knockdown (KD) 293T cells, using [KD Validated] MAD1/MAD1L1 Rabbit pAb (CAB22000) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
