The [KD Validated] PHF11 Monoclonal Antibody (CAB23994) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in mice, exhibits strong reactivity with human samples and has been rigorously validated for use in immunofluorescence, immunohistochemistry, and flow cytometry applications.PHF11, also known as plant homeodomain finger protein 11, is known to play a role in gene regulation and cell differentiation. Its involvement in chromatin remodeling and transcriptional activation makes it a key target for investigating pathways related to development, cell growth, and differentiation. The PHF11 Monoclonal Antibody enables precise detection and visualization of PHF11 protein expression in different cell types and tissues, making it an essential tool for research in the fields of molecular biology and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KD Validated] PHF11 Monoclonal Antibody
SKU:
CAB23994
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC62550
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51131
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using [KD Validated] PHF11 Rabbit mAb (CAB23994) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Rat spleen using [KD Validated] PHF11 Rabbit mAb (CAB23994) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.