Western blot analysis of extracts of various cell lines, using TOLLIP antibody (CAB21551) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] TOLLIP Polyclonal Antibody (CAB21551) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the Tollip protein, this antibody allows for the detection and analysis of Tollip in various cell types, making it ideal for studies in immunology, inflammation, and infectious disease research.Tollip is a key player in the regulation of toll-like receptor signaling pathways, which are essential for initiating immune responses to invading pathogens.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KD Validated] TOLLIP Polyclonal Antibody
SKU:
CAB21551
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL-1RAcPIP, [KD Validated] TOLLIP
Positive Sample:
293T, SW620, Mouse testis, Mouse brain, Rat testis
Cellular Localization:
Cytoplasm.
Calculated MW:
30kDa
Observed MW:
30kDa
This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
54472
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KD Validated] TOLLIP Rabbit pAb (CAB21551) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3S.
Western blot analysis of lysates from wild type (WT) and TOLLIP knockdown (KD) 293T cells, using [KD Validated] TOLLIP Rabbit pAb (CAB21551) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3S.
