The KDM2A Antibody (CAB18636) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, demonstrates high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By binding to the KDM2A protein, this antibody enables accurate detection and analysis in various cell types, providing insights into the role of KDM2A in cellular processes.KDM2A, also known as lysine-specific demethylase 2A, is a key player in chromatin remodeling and transcriptional regulation, impacting cell proliferation, differentiation, and development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
KDM2A Antibody
SKU:
CAB18636
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FBL7, CXXC8, FBL11, FBXL11, JHDM1A, LILINA, KDM2A
Positive Sample:
HeLa, BT-474, Jurkat, Mouse liver
Cellular Localization:
Nucleus, Nucleoplasm.
Calculated MW:
133kDa
Observed MW:
126kDa
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
22992
RRID
AB_2862374
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KDM2A Rabbit pAb (CAB18636) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
