Anti-KIF14 Antibody (CAB10275)

Antibody Name:	Anti-KIF14 Antibody
Antibody SKU:	CAB10275
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1399-1648 of human KIF14 (NP_055690.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HL-60

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1399-1648 of human KIF14 (NP_055690.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LENG NNKA ASVQ EEFM DAVC DGVG LGMK ILLD SGLE KAKE LQHE LFRQ CTKN EVTK EMKT NAMG LIRS LENI FAES KIKS FRRQ VQEE NFEY QDFK RMVN RAPE FLKL KHCL EKAI EIII SALK GCHS DINL LQTC VESI RNLA SDFY SDFS VPST SVGS YESR VTHI VHQE LESL AKSL LFCF ESEE SPDL LKPW ETYN QNTK EEHQ QSKS SGID GSKN KGVP KRVY ELHG SSPA VSSE ECTP SRIQ WV
Gene ID:	9928
Uniprot:	Q15058
Cellular Location:	Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle
Calculated MW:	186kDa
Observed MW:	190kDa

Synonyms:

KIF14, MKS12

Background:

This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	KIF14: Plays an essential role in cytokinesis. Belongs to the kinesin-like protein family.
UniProt Protein Details:	Protein type:Microtubule-binding; Motor Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: cytosol; kinesin complex; membrane; microtubule; midbody; plasma membrane; spindle midzone Molecular Function:ATP binding; ATPase activity; microtubule binding; microtubule motor activity; PDZ domain binding; plus-end-directed microtubule motor activity; protein binding; protein kinase binding; tubulin binding Biological Process: activation of protein kinase activity; cell proliferation in forebrain; cerebellar cortex development; cerebellar granular layer structural organization; cerebellar Purkinje cell layer structural organization; cerebral cortex development; establishment of protein localization; hippocampus development; microtubule depolymerization; microtubule-based movement; mitotic metaphase plate congression; negative regulation of apoptosis; negative regulation of integrin activation; negative regulation of neuron apoptosis; olfactory bulb development; positive regulation of cell proliferation; positive regulation of cytokinesis; proteasomal ubiquitin-dependent protein catabolic process; regulation of cell adhesion; regulation of cell growth; regulation of cell migration; regulation of G2/M transition of mitotic cell cycle; regulation of myelination; regulation of neuron apoptosis; regulation of Rap protein signal transduction; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Disease: Meckel Syndrome 12
NCBI Summary:	This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
UniProt Code:	Q15058
NCBI GenInfo Identifier:	23396633
NCBI Gene ID:	9928
NCBI Accession:	Q15058.1
UniProt Secondary Accession:	Q15058,Q14CI8, Q4G0A5, Q5T1W3,
UniProt Related Accession:	Q15058
Molecular Weight:	186kDa
NCBI Full Name:	Kinesin-like protein KIF14
NCBI Synonym Full Names:	kinesin family member 14
NCBI Official Symbol:	KIF14
NCBI Official Synonym Symbols:	MKS12
NCBI Protein Information:	kinesin-like protein KIF14
UniProt Protein Name:	Kinesin-like protein KIF14
Protein Family:	Kinesin-like protein
UniProt Gene Name:	KIF14

	Western blot analysis of extracts of various cell lines, using KIF14 antibody (CAB10275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat ovary using KIF14 antibody (CAB10275) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using KIF14 antibody (CAB10275) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using KIF14 Polyclonal Antibody (CAB10275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using KIF14 Polyclonal Antibody (CAB10275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)