Anti-KIR3DL3 Antibody (CAB10064)

Antibody Name:	Anti-KIR3DL3 Antibody
Antibody SKU:	CAB10064
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-322 of human KIR3DL3 (NP_703144.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, SGC-7901

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-322 of human KIR3DL3 (NP_703144.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QDKP FLSA WPGT VVSE GQHV TLQC RSRL GFNE FSLS KEDG MPVP ELYN RIFR NSFL MGPV TPAH AGTY RCCS SHPH SPTG WSAP SNPV VIMV TGVH RKPS LLAH PGPL VKSG ETVI LQCW SDVR FERF LLHR EGIT EDPL RLVG QLHD AGSQ VNYS MGPM TPAL AGTY RCFG SVTH LPYE LSAP SDPL DIVV VGLY GKPS LSAQ PGPT VQAG ENVT LSCS SRSL FDIY HLSR EAEA GELR LTAV LRVN GTFQ ANFP LGPV THGG NYRC FGSF RALP HAWS DPSD PLPV SVTG NSRN L
Gene ID:	115653
Uniprot:	Q8N743
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	44kDa
Observed MW:	45kDa

Synonyms:

KIR3DL3, CD158Z, KIR3DL7, KIR44, KIRC1

Background:

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several 'framework' genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the 'framework' loci that is present on all haplotypes.

UniProt Protein Function:	Receptor on natural killer cells. May inhibit the activity of NK cells thus preventing cell lysis.
NCBI Summary:	Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8N743
NCBI GenInfo Identifier:	269849611
NCBI Gene ID:	115653
NCBI Accession:	Q8N743.2
UniProt Secondary Accession:	Q8N743,A9PL62, A9PL64, A9PL65, A9PL66, A9PL67, A9PL68 A9PZV4, A9PZV7, A9PZV8, A9Q066, A9Q0R5,
UniProt Related Accession:	Q8N743
Molecular Weight:	44,928 Da
NCBI Full Name:	Killer cell immunoglobulin-like receptor 3DL3
NCBI Synonym Full Names:	killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 3
NCBI Official Symbol:	KIR3DL3
NCBI Official Synonym Symbols:	KIR44; KIRC1; CD158Z; KIR3DL7
NCBI Protein Information:	killer cell immunoglobulin-like receptor 3DL3
UniProt Protein Name:	Killer cell immunoglobulin-like receptor 3DL3
UniProt Synonym Protein Names:	CD158 antigen-like family member Z; Killer cell inhibitory receptor 1; CD_antigen: CD158z
UniProt Gene Name:	KIR3DL3

	Western blot analysis of extracts of various cell lines, using KIR3DL3 antibody (CAB10064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat heart using KIR3DL3 antibody (CAB10064) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using KIR3DL3 antibody (CAB10064) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using KIR3DL3 antibody (CAB10064) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using KIR3DL3 antibody (CAB10064) at dilution of 1:100 (40x lens).

Antibodies

KIR3DL3 Antibody (PACO01975)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)