Anti-KISS1R Antibody (CAB2967)

Antibody Name:	Anti-KISS1R Antibody
Antibody SKU:	CAB2967
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human KISS1R

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480

Immunogen:	A synthetic peptide of human KISS1R
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	84634
Uniprot:	Q969F8
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	42kDa
Observed MW:	43kDa

Synonyms:

KISS1R, AXOR12, CPPB1, GPR54, HH8, HOT7T175, KISS-1R

Background:

The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty.

UniProt Protein Function:	KISS1R: Receptor for metastin (kisspeptin-54 or kp-54), a C- terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine- tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins. Defects in KISS1R are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Defects in KISS1R are a cause of central precocious puberty (CEPREPU). Precocious puberty is defined as the development of secondary sexual characteristics before the age of 8 years in girls and 9 years in boys. Central precocious puberty refers to a gonadotropin-dependent type which results from premature activation of the hypothalamic-pituitary-gonadal axis. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:	Protein type:Cell cycle regulation; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: integral to membrane; integral to plasma membrane; intracellular membrane-bound organelle; plasma membrane Molecular Function:neuropeptide receptor activity; protein binding Biological Process: G-protein signaling, coupled to IP3 second messenger (phospholipase C activating) Disease: Hypogonadotropic Hypogonadism 8 With Or Without Anosmia; Precocious Puberty, Central, 1
NCBI Summary:	The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty. [provided by RefSeq, Jul 2008]
UniProt Code:	Q969F8
NCBI GenInfo Identifier:	125987836
NCBI Gene ID:	84634
NCBI Accession:	Q969F8.2
UniProt Secondary Accession:	Q969F8,Q96QG0, A5D8U2, B2RTV1,
UniProt Related Accession:	Q969F8
Molecular Weight:	42,586 Da
NCBI Full Name:	KiSS-1 receptor
NCBI Synonym Full Names:	KISS1 receptor
NCBI Official Symbol:	KISS1R
NCBI Official Synonym Symbols:	HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
NCBI Protein Information:	kiSS-1 receptor
UniProt Protein Name:	KiSS-1 receptor
UniProt Synonym Protein Names:	G-protein coupled receptor 54; G-protein coupled receptor OT7T175; hOT7T175; Hypogonadotropin-1; Kisspeptins receptor; Metastin receptor
Protein Family:	KiSS-1 receptor
UniProt Gene Name:	KISS1R
UniProt Entry Name:	KISSR_HUMAN

	Western blot analysis of extracts of SW480 cells, using KISS1R antibody (CAB2967) at 1:350 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using KISS1R Antibody (CAB2967) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human B cell lymphoma using KISS1R Antibody (CAB2967) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using KISS1R Antibody (CAB2967) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
KISS1R Antibody (PACO05528)	Human KiSS1R / GPR54 ELISA Kit
KISS1R Antibody (PACO10116)	Human KiSS-1 receptor (KISS1R) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)