Anti-KLF8 Antibody (CAB16321)

Antibody Name:	Anti-KLF8 Antibody
Antibody SKU:	CAB16321
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-280 of human KLF8 (NP_009181.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, 293T, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-280 of human KLF8 (NP_009181.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GKNA GSVK VDPT SMSP LEIP SDSE ESTI ESGS SALQ SLQG LQQE PAAM AQMQ GEES LDLK RRRI HQCD FAG
Gene ID:	11279
Uniprot:	O95600
Cellular Location:
Calculated MW:	26kDa/27kDa/30kDa/39kDa
Observed MW:	45kDa

Synonyms:

KLF8, BKLF3, ZNF741

Background:

This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	KLF8: Transcriptional repressor and activator. Binds to CACCC- boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). Belongs to the Sp1 C2H2-type zinc-finger protein family.
UniProt Protein Details:	Protein type:C2H2-type zinc finger protein; Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: Xp11.21 Cellular Component: nucleus Molecular Function:DNA binding; metal ion binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Code:	O95600
NCBI GenInfo Identifier:	17366251
NCBI Gene ID:	11279
NCBI Accession:	O95600.2
UniProt Related Accession:	O95600
Molecular Weight:	Calculated: 26kDa; 27kDa; 30kDa; 39kDaObserved: 45kDa
NCBI Full Name:	Krueppel-like factor 8
NCBI Synonym Full Names:	Kruppel like factor 8
NCBI Official Symbol:	KLF8
NCBI Official Synonym Symbols:	BKLF3; ZNF741
NCBI Protein Information:	Krueppel-like factor 8
UniProt Protein Name:	Krueppel-like factor 8
UniProt Synonym Protein Names:	Basic krueppel-like factor 3; Zinc finger protein 741
Protein Family:	Krueppel-like factor
UniProt Gene Name:	KLF8
UniProt Entry Name:	KLF8_HUMAN

	Western blot analysis of extracts of various cell lines, using KLF8 antibody (CAB16321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of A431 cells using KLF8 antibody (CAB16321) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using KLF8 antibody (CAB16321) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

KLF8 Antibody (PACO03390)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)