The AES Antibody (CAB7874) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, created using rabbit immunization, shows high reactivity with both human and mouse samples, making it versatile for a wide range of research applications.Validated for use in Western blotting, this antibody binds specifically to the AES protein, facilitating accurate detection and analysis in different cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
AES Antibody
SKU:
CAB7874
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AES, GRG, ESP1, GRG5, AES-1, AES-2, Grg-5
Positive Sample:
A-549, HeLa, U-251MG, Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
22kDa
Observed MW:
22kDa
The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
166
RRID
AB_2768272
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AES Rabbit pAb (CAB7874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
